Analysis Note

ControlK562 cell lysate

Application

Research Sub CategoryRNA Metabolism & Binding Proteins

Research CategoryEpigenetics & Nuclear Function

Use Anti-hnRNP F Antibody, clone 3H4 (Mouse Monoclonal Antibody) validated in WB, IP, ICC to detect hnRNP F also known as heterogeneous nuclear ribonucleoprotein F.

Immunoprecipitation Analysis:
A representative lot was used by an independent laboratory for IP. (Matunis, M.J., et al. (1994). Nucleic Acids Res. 22(6):1059–1067.)

Immunofluorescence Analysis:
A representative lot was used by an independent laboratory in IF. (Matunis, M.J., et al. (1994). Nucleic Acids Res. 22(6):1059–1067.)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The hnRNP F protein is member of a subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs).
The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and associate with pre-mRNAs in the nucleus. These complexes are associated with pre-mRNA processing and other aspects of mRNA metabolism and transport. There are more than 20 different heterogeneous nuclear ribonucleoproteins (hnRNPs) associated with pre-mRNAs in the nucleus of mammalian cells. These different hnRNPs display a range of affinities for a variety of ribonucleotidehomopolymers and ssDNA sequences. In constrast, hnRNP F protein as well as hnRNP H (a highly related protein) appear to bind only to poly(rG). These unique RNA-binding properties, as well as the amino acid sequence and other data showing a distinct intranuclear localization of hnRNP F and hnRNP H suggest they are unique from other hnRNP and play an important role in the processing of RNAs containing guanosine-rich sequences.

Immunogen

Epitope: Unknown

Recombinant protein corresponding to human hnRNP F.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in K562 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected hnRNP F on 10 µg of K562 cell lysate.

Specificity

This antibody recognizes hnRNP F.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

Calculated: 46 kDa Observed: ~53 kDa